Psichecktm-2 vector
WebMyocyte enhancer factor (MEF2) is a family of transcrip- tion factors that binds to an A/T-rich DNA element in the promoter regions of muscle-specificgenes(12). MEF2 exerts a diversity of function during myogenic differentiation and is involved in the control of various aspects of muscle fiber properties (13). WebMar 26, 2024 · The pGL3.0 luciferase reporter vector (E1910; Promega, Madison, WI) and psiCHECKTM-2 vector (C8021, Promega) were used for the dual-luciferase reporter assay to verify the binding sites in sequences under study for Sp1 and miR-375, respectively.
Psichecktm-2 vector
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WebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … http://www.youbio.cn/product/vt1400
http://www.kelei-biology.com/plus/view.php?aid=1361 Webinserted into psiCHECKTM-2 Vector (Promega, Madison, WI, USA) to construct plasmids used in the following dual-luciferase reporter assays. The open reading frame (ORF) of HMGB1 was cloned and inserted into pCMV6AC-GFP (Origene, - Rockville, MD, USA) vector to generate HMGB1. The primer sequences were listed in Supplementary Table 5.
WebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格： ¥0. 货 号： 3544763. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！. WebHEK293T cells were transfected with psiCHECKTM-2-Wt-IGF2BP2 or psiCHECKTM-2-mut-IGF2BP2, together with miR-138 mimics, inhibitor or the negative control (GenePharma, China) by Lipofectamine 2000 (Invitrogen, USA). Forty- eight hours later, relative luciferase activities were assessed using the Dual-Luciferase Reporter Assay System (Promega, USA).
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WebApr 15, 2024 · Fig. 2: Néel vector control by cooling in opposite magnetic fields. a Optical image and its MCD image at 280 K of the front ab plane in a 500 μm-thick crystal. buggies for 3 to 4 year oldsWebif the psiCHECK™-2 Vector could report a known miR-21 target (miR-21 mismatch; 5). Complementary oligonucleotides containing the exact miR-21 sequence or a mismatched miR-21 target (5) were generated with flanking partial SgfI and PmeI restriction sites (Table 2). A restriction site not found in the psiCHECK™-2 Vector was also included to crossbody taschen damenWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … buggies for disabled childrenWebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were … buggies for 5 year oldsWeb(Chuzhou, Anhui, China) and inserted into the psiCHECKTM-2 vector (Promega, Madison, WI). HEK293T cells were seeded in 24-well plates, and Lipofectamine 2000 was used to … buggies for 3 year oldsWebMar 20, 2024 · The psiCHECKTM-2 vector with the cloned miR-200c binding site of XIST, named XIST-wild type (WT), was co-transfected with miR-200c mimics or mimics NC using the Lipofectamine 2000 transfection reagent. The mutation-carrying psiCHECKTM-2 vector with a different sequence of the miR-200c binding site, named as the XIST-mutation (Mut), … buggies from birthWeb2 days ago · WT and mutant PABPC1L 3′UTR were cloned into the C-terminus of the Renilla luciferase gene in the psiCHECK™-2 vector. Empty vector was used as the control, and Firefly luciferase expression served as the internal standard for transfection efficiency. HeLa cell extracts were prepared 36 h after transfection and assayed for the Firefly and ... buggies gas station