SpletDescription; Overview: The pCDF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag that results in native … Splet01. sep. 2024 · The E. coli strains and plasmids used in this study are listed in Table 1.DH5α and BL21 Star (DE3) cells (Thermo Fisher) were used as host strains for routine cloning experiments. Genes plsC and galU were obtained from E. coli JM109 (Promega), plsB was from E. coli MG1655 (Genbank accession NC_000913) and E. coli BL21 Star (DE3) …
A semisynthetic organism engineered for the stable expansion of …
Splet15. jul. 2011 · Rad6b/Rad18 complexes were co-expressed via the pCDF1b/pET28a vectors and first purified via a His 6 tag on Rad18 using TALON beads (Clontech), eluting with 200 mM imidazole. Initial purification was via a 6-mL Heparin column (GE Healthcare), with a step elution into a buffer containing 440 mM NaCl, followed by gel filtration with an S200 … Splet10. maj 2013 · The pCDF1b vector was digested with the restriction enzymes XhoI and NdeI, which eliminated the sequence encoding the His6 tag from the vector backbone, thereby … crimini internazionali esempi
Functional Annotation and Molecular Cloning of Gene of Interest
SpletMillipore expression vector pcdf 1b Expression Vector Pcdf 1b, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more SpletThe pCDF1b vector was digested with the restriction enzymes XhoI and NdeI, which eliminated the sequence encoding the His6 tag from the vector backbone, thereby … Splet17. jul. 2024 · A single colony was then used to inoculate 5 mL of LB medium containing 50 μg/mL streptomycin (only where the pCDF1b-Sfp vector was present) and kanamycin, … crimini irrisolti america