2024 Maize transformation protocol

Maize transformation protocol

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August undefined, 2024

WebThis chapter describes two transformation protocols using Agrobacterium- and biolistic-mediated methods for gene delivery. Immature zygotic embryos of maize inbred B104, …

An Improved Agrobacterium-Mediated Transformation and …

WebMay 4, 2024 · An Improved Agrobacterium -Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature Embryos … Web15 hours ago · Maize straw returning is one of the important measures to improve dryland soil organic carbon (SOC). However, the effects of different maize parts on SOC fractions with different soil fertility levels in situ are not exactly clear. Therefore, an in situ field incubation experiment over 540 days, by adding different 13C-labeled maize parts (root, … iactionresult for error

Biolistic gun-mediated maize genetic transformation - PubMed

WebJul 27, 2024 · For maize Lc gene expression analysis, total RNA was extracted from young shoots of putative transgenic and untransformed seedlings using Qiagen RNeasy Plant Mini Kit (Qiagen, China), and the … WebLH244 (inbred) Maize Transformation/Editing services are now available! Using a modified B104 protocol with agrobacterium, we are able to transform immature embryos into this … WebMar 1, 2024 · Maize is an important crop for billions of people globally. The existing immature embryo-based regeneration protocol of maize has major limitations due to the non-availability of explants... iactionresult and actionresult

Agrobacterium-mediated transformation of maize

Frontiers An Improved Agrobacterium-Mediated Transformation …

WebIn this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature embryo-derived callus cultures can be transformed using this protocol. WebSep 29, 2024 · Maize kernel development can be divided into three phases: early development, filling and maturation 10. At an early stage, the maternal nucellar tissue degenerates by programmed cell death... molson coors milwaukee breweryWebIn this chapter, we describe the Agrobacterium-mediated maize transformation protocol used routinely in our laboratory ( 6) to transform immature embryos of the maize Hi II genotype. A. tumefaciens strain EHA101 harboring a standard binary vector is used to infect the embryos and deliver transgenes into plant cells. iactionresult onget

"WebFeb 14, 2024 · Demonstrated here is a detailed protocol for Agrobacterium-mediated genetic transformation of maize inbred lines using morphogenic genes Baby boom (Bbm) and Wuschel2 (Wus2). Bbm is regulated by the maize phospholipid transferase gene (Pltp) promoter, and Wus2 is under the control of a maize auxin-inducible (Axig1) promoter. " - Maize transformation protocol

Maize transformation protocol

Crop improvement through genetic engineering: development of ...

WebDec 28, 2024 · This collection highlights research protocols designed to guide users on the use of maize for research purposes (including DNA isolation, in situ hybridization, chromosome preparation, and more). ... Morphogenic Regulator-Mediated Transformation of Maize Inbred B73. Muruganantham Mookkan, Kimberly Nelson-Vasilchik, Joel Hague, … WebNov 11, 2024 · We have recently described a transformation method using slices of the embryo axis from mature maize seed as a starting explant for Agrobacterium -mediated transformation . This is facilitated by the maize transcription factors Bbm and Wus2 (morphogenic genes ) which act synergistically to stimulate embryogenic growth [ 2 ].

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WebDec 15, 2024 · Indeed, preliminary data suggest that a higher efficiency of maize embryo transformation is achievable using a combination of the wheat GRF–GIF chimaera and the maize BBM transcriptional ... WebJan 1, 2003 · In general, maize transformation protocols use culture media prepared with N6 or MS salts (Frame et al., 2006). The protocols can be optimized by altering the …

WebMay 4, 2024 · An Improved Agrobacterium -Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature Embryos Front Plant Sci. 2024 May 4;13:860971. doi: 10.3389/fpls.2024.860971. eCollection 2024. Authors Minjeong Kang 1 2 3 , Keunsub Lee 1 2 , Todd Finley 4 , Hal Chappell 4 , Veena Veena … WebThe transfection protocol describes the use of a dual-expression vector that carries the coding sequence for two fluorescent proteins (FPs), one driven by a constitutive promoter for normalization for transfection efficiency and the other driven by the construct of interest.

WebNational Center for Biotechnology Information WebDec 21, 2024 · Little is known about the fate of heavy metals in the recycling system of animal manure–black soldier fly larvae (BSFL) transformation-larval frass application. In this work, BSFL-transformed pig manure with different concentrations of exogenous cadmium (Cd) (0, 3, 15, 30 mg kg−1), and the obtained BSFL frass fertilizer were further …

WebJun 9, 2024 · Maize functional genomics research and genetic improvement strategies have been greatly accelerated and refined through the development and utilization of genetic …

WebProtocol TD-P Revision 3.0 Creation Date: 5/25/2016 Revision Date: 12/3/2024 Stable Maize Transformation Protocol Adapted from Raizada and Walbot, Stanford University (2001) Introduction Biolistic transformation is a widely used method for introducing genes into plants that are resistant to Agrobacterium-mediated transformation. This technique ... iactionresult interfaceWebWang K, Frame B (2009) Biolistic Gun-Mediated Maize Genetic Transformation. In: Scott MP (ed) Transgenic maize: methods and protocols. Humana, Totowa, NJ, pp 29–45 Vain P, McMullen MD, Finer JJ (1993) Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize. Plant Cell Rep 12:84–88 molson coors milwaukee corporate addressWebexpression cassettes have led to the development of reliable transformation protocols for major cereals, including maize. Consequently, this research group has focused its … iactionresult not authorizedWebMaize transformation, which is a prerequisite for genetically modified maize development, is no longer a major bottleneck. Protocols using morphogenic regulators have evolved significantly... iactionresult in c#WebDec 1, 2024 · Genotype dependence in plant transformation means that one can only achieve genetic transformation on limited numbers of plant species or varieties. In many instances, a successful protocol for one plant variety may not be transferrable to another one. For example, the protocol for maize inbred B104 cannot be applied to B73 [5]. … iactionresult onpostWebThis study established a new Agrobacterium-mediated maize transformation protocol using a standard binary vector system. The resulting transgenic maize plants then were evaluated. Results showed that transformants generated from this new method have better qualities compared with those obtained from particle bombardment transformation.;This ... iactionresult in mvcWebOct 22, 2024 · Maize B73 is a reference genome and has long been a major resource for genetics and molecular biology research. We have developed an efficient B73 … iactionresult in webapi