WebApr 29, 2024 · For RNA interference, siRNAs for DHHC4, DHHC5, and DHHC6 were synthesized from GenePharma Corporation; siRNAs for SelK and Cav-1 were obtained from Santa Cruz Biotechnology. RAW264.7 cells at 60% confluence in a 6-well plate were transfected with specific siRNA oligonucleotides (50 nM) using Lipofectamine RNAiMAX … WebArticle Snippet: Interfering RNA (shRNA) oligonucleotides were annealed and cloned into the pGPU6-GFP-Neo knockdown vector (C02007, Genepharma Corporation, …
NF-κB suppresses apoptosis and promotes bladder cancer cell
WebDec 2, 2024 · MiR-2682-5p mimics and a miR-2682-5p mimic NC were constructed by GenePharma Corporation. After 2 days, transfected cells were collected, and at last RT-qPCR was adopted to test the transfection efficacy. CCK-8. PANC-1 or BxPC-3 cells (1 × 10 3 cell/well) were plated in 96-well plates, and then incubated for 24 h. Cellular … WebGenePharma Co., Ltd (NEEQ:430601) is a biotechnology company that researches and develops RNA based technologies and products headquartered in Suzhou, with four … optical recruitment agency
MicroRNA-34a suppresses invasion and metastatic in
WebSep 20, 2024 · The siRNA was constructed by GenePharma Corporation and transfected according to the experimental instructions. The siRNA‐kindlin‐2:5′‐GCU UCC CAA CAU GAA GUA UTT‐3′ and 5′‐AUA CUU CAU GUU GGG AAG CTT‐3′ (GenePharma) was used to deplete the expression of kindlin‐2 cells and a siRNA‐negative control: 5′‐UUC UCC … WebAug 21, 2024 · COLEC12 knockdown via lentivirus (sh‐COLEC12), TLR4 knockdown via lentivirus (sh‐TLR4), and a control lentivirus were designed and chemically synthesized by GenePharma Corporation, in Shanghai, China. The lentivirus vectors were stored at −80°C, accordingly. WebMar 21, 2024 · Human small interfering RNA (siRNA) and scrambled siRNA were chemically synthesized by Shanghai GenePharma Corporation (SGC, shanghai, China). The cells were transfected with 20 μM synthesized siRNA targeting human SIRT6, and scrambled siRNA was used as a negative control (NC siRNA). The siRNA and transfection reagent … portland balaji temple