WebA systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using … WebJan 1, 2016 · Total lipid extracts can be either analyzed directly or fractionated to isolate wax monoesters for HPLC analysis. Normal-phase liquid chromatography is commonly used for the isolation of wax monoesters. ... The experimental conditions for Ag-HPLC: two columns ChromSpher Lipids (250 × 4.6 mm, 5 μm particles) in series at 35 °C, …
Retention of lipids in silver ion high-performance liquid ...
WebAug 13, 2004 · Two Varian ChromSpher lipids columns connected in series and isocratic solvent systems of 1.0% or 1.5% acetonitrile (ACN) in hexane were used to analyze FAMEs (zero to six double bonds; cis/trans isomers), TAGs (homogeneous (triacetyl-, tristearoyl-, trioleoyl-, trilinoeyl- and trilinolenoyl-glycerols) and positional … WebOct 1, 2013 · Isobaric triacylglycerols which differ in the sn‐1(3) and sn‐2 positions of acyl chains are regioisomers. The objective of this review is to describe the resolution of regioisomers by silver‐ion chromatography, RP–HPLC, and by GLC. Furthermore, the aim of this review is to discuss the determination of regioisomers solely by mass spectrometry … poynton rooms
Chromspher Lipids, Valco, 120 A, 5 um, 4.6 x 250 mm, 1/pk
WebVarian ChromSpher Lipids columns are packed with a cation and altered selectivity. exchange resin in the Ag+ ionic form. It is specifically designed We recommend a Varian Cation Exchange High Efficiency. for the separation of triglycerides. guard column (Part No. CP28089). Replacement of the guard column is required when increased. WebMar 14, 1997 · To improve peak resolutions and to allow the injection of larger sample sizes, two ChromSpher Lipids columns (Cat. No. 28313; 250 mm×4.6 mm I.D. stainless steel; 5 μm particle size; silver ion impregnated; Chrompack) were connected in series. Solvent flow was standardized at 1.0 ml/min. 2.3. Analyses of HPLC fractions WebAbstract A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using the most widespread hexane- and dichloromethane-based mobile phases. hansa tools